REBECCA GRAY

Address: 1376 Mowry Road
PO Box 103610
University of Florida
Gainesville, FL 32610-3610
Lab: 415 Genetics Institute-7305

Tel: (352) 273-8091

Email: rgray@ufl.edu

Education:

2006 Passed qualifying exams for Ph.D. Candidacy

2005 M.A., Anthropology University of Florida, Gainesville, FL

2001 B.A., Anthropology Millersville University, Millersville PA

Research Appointments:

Fall 2005-present: Research Assistantship under Dr. Connie Mulligan, Dept of Anthropology

Fall 2004: Research Assistantship under Dr. David Daegling, Dept of Anthropology

Teaching Appointments:

Spring 2005: Teaching Assistantship for Introduction to Physical Anthropology

Summer 2004: Teaching Assistantship for Human Sexuality

On-going projects

Genetic analysis of variants contributing to alcohlism in Native American populations

· Conducted high through-put genetic analysis of 13 SNPs and 1 STR from the alpha synuclein gene in Dr. Andrew Singleton’s laboratory at NIH/NIAAA in April, 2005

o Performing statistical analyses on data to determine significant association with alcoholism

· Finishing SNP typing of ADH gene in Plains population

Past projects:

Phylogenetic Analysis of Syphilis (Treponema pallidum) Sequences

• Used sequence data from tpr genes generated by colleagues at the University of Washington, Seattle

· Analyzing phylogenies for several loci to determine evolutionary history of the tpr gene family and subspecies of T. pallidum organisms

Genetic variation at the mitochondrial 9-bp repeat locus in the Sakha of Siberia

• Master’s Research in the Laboratory of Dr. Mulligan
• Research involved PCR analysis and genetic sequencing of mtDNA from Siberian samples

Other Lab Experience

· Spring 2005-present: Conducting research in the laboratory of Dr. Maureen Goodenow, Department of Pathology

o Sequence determination of drug-resistant mutations in response to efavirenz and nelfinavir drug treatments in juvenile HIV+ patients

o Isolating viral RNA from patients’ plasma, conducting PCR, ligation-transformation, and processing of samples for genetic sequencing

· Spring 2003-Spring 2004: Conducted research in the laboratory of Dr. Joyce Koenig, Department of Neonatology

o Separated neutrophils and CD34+ cells from cord and adult blood, isolated RNA from cells, performed real-time PCR on samples

· Fall 2002-Spring 2003: Assisted in research in the laboratory of Ginger Clark, ICBR

o Isolation of DNA; PCR and gel analysis

Publications

R.R. Gray, C.J. Mulligan, B.J. Molini, E.S. Sun, L. Giacani, C. Godornes, A. Kitchen, S.A. Lukehart, and A. Centurion-Lara. 2006. Molecular Evolution of the tprC, D, I, K, G, and J Genes in the Pathogenic Genus Treponema. Mol Biol Evol in press.

L. Tarskaia, R.R. Gray, B. Burkley, C.J. Mulligan. 2006. Genetic variation at the mitochondrial 9-bp repeat locus in the Sakha of Siberia. Human Biology 78:179-198.

Graduate Committee Members:

Connie J. Mulligan, Ph.D., Anthropology Department, chair

John Krigbaum, Ph.D., Anthropology Department

David Reed, Ph.D., Museum of Natural History

Marta L. Wayne, Ph.D., Zoology Department

Rong-ling Wu, Ph.D., Statistics Department

CONNIE J. (KOLMAN) MULLIGAN

A. Biographical sketch

Address: Dept of Anthropology

1112 Turlington Hall

University of Florida

Gainesville, FL 32611-7305

Tel: 352-392-2253, ext. 248, Fax: 352-392-6929

Email: mulligan@anthro.ufl.edu

Education

1990 Ph.D., Yale University, New Haven, CT, Molecular Biophysics and Biochemistry

1985 M.Phil., Yale University, New Haven, CT, Molecular Biophysics and Biochemistry

1983 B.S., Honors Biology and Chemistry, University of Illinois, Champaign-Urbana, IL

Appointments

2005 – present Associate professor, Dept. of Anthropology, University of Florida

2003 - present Associate director, University of Florida Genetics Institute

1999 - 2005 Assistant professor, Dept. of Anthropology, University of Florida

1998-2000 Senior research associate, National Institute on Alcohol Abuse and Alcoholism

1996-1998 Research biologist, Smithsonian Center for Materials Research and Education

1995-1996 Research associate, Smithsonian Center for Materials Research and Education

1991-1995 Postdoctoral fellow, Smithsonian Tropical Research Institute

1990-1991 Postdoctoral fellow, Molecular Biophysics and Biochemistry, Yale University

B. Five publications related to this work

Mulligan, CJ, Hunley, K, Cole, S, Long, JC. 2004. Population genetics, history, and health patterns in Native Americans, Annu Rev Genomics Hum Genet, 5:295-315.

Sun, G, McGarvey ST, Bayoumi R, Mulligan CJ, Barrantes R, Raskin S, Zhong Y, Akey J, Chakraborty R, Deka R. 2003. Global genetic variation at nine short tandem repeat loci and implications on forensic genetics, Eur J Hum Genet, 11:39-49.

Kolman, CJ and Bermingham, E. 1997. Mitochondrial and nuclear DNA diversity in the Choco and Chibcha Amerinds of Panama, Genetics, 147: 1289-1302.

Kolman, CJ, Sambuughin, N and Bermingham, E. 1996. Mitochondrial DNA analysis of Mongolian populations and implications for the origin of New World founders, Genetics, 142: 1321-1334.

Kolman, CJ, Bermingham, E, Cooke, R, Ward, RH, Arias, T and Guionneau-Sinclair, F. 1995. Reduced mtDNA diversity in the Ngobe Amerinds of Panama, Genetics, 140:275-283.

Five other publications

Mulligan CJ, 2005, Isolation and analysis of DNA from archaeological, clinical, and natural history specimens, Meth Enzymol, 395:87-104.

Mulligan CJ, Robin RW, Osier MV, Kidd KK, Sambuughin N, Goldfarb LG, Kittles RA, Goldman D, Long JC. 2003. Complex association and linkage of alcohol metabolism genes (ADH2, ADH3, ALDH2) and vulnerability of alcoholism in an American Indian population, Hum Genet, 113:325-336.

Kolman, CJ and Tuross, N. 2000. Ancient DNA analysis of human populations, Am J Phys Anthropol, 111: 5-23.

Batista, O, Kolman, CJ, Bermingham, E. 1995. Mitochondrial DNA diversity in the Kuna Amerinds of Panama. Hum Mol Genet, 4:921-929.

Ollis, D, Kline (Kolman), CJ and Steitz, TA. 1985. Domain of Escherichia coli DNA polymerase showing sequence homology to T7 DNA polymerase, Nature, 313: 818-819.

C. Synergistic activities

- Associate Director of UF Genetics Institute, an interdisciplinary genetics research institute combining the College of Liberal Arts and Science, College of Medicine, and Institute of Food and Agriculture Science

- Developing bioinformatics initiatives and collaborations at UF. Helped organize UF Genetics Institute/College of Engineering Bioinformatics Workshop, Sept 16, 2004. Co-organizer of Florida Bioinformatics Workshop 2005, Feb 21-22, 2005.

- Developed core series of graduate level laboratory courses for UF Department of Anthropology, developed and taught laboratory course on molecular genetics, developed webpages for courses on molecular genetics of disease and molecular anthropology

- Active member of American Association for Physical Anthropologists (co-organized two symposia at AAPA meetings), Research Society on Alcoholism, American Association for the Advancement of Science, American Association of University Women (obtained funding for one international post-doctoral associate)

D. Collaborators

Joseph Akey - Center for Genome Information, University of Cincinnati

Ramiro Barrantes - Instituto de Investigaciones en Salud, Universidad de Costa Rica

Riad Bayoumi - Department of Biochemistry, Sultan Qaboos University, Muscat, Oman

Eldredge Bermingham - Smithsonian Tropical Research Institute, Smithsonian Institution

Ranajit Chakraborty - Center for Genome Information, University of Cincinnati

Suzanne Cole - Department of Human Genetics, University of Michigan Medical School

Ranjan Deka - Center for Genome Information, University of Cincinnati

Lev G. Goldfarb - National Institute of Neurological Disorders and Stroke, NIH

David Goldman - National Institute on Alcohol Abuse and Alcoholism, NIH

Diane Hesselbrock - Department of Surgery, Washington University

Keith Hunley - Department of Anthropology, University of New Mexico

Rick A. Kittles - National Human Genome Center at Howard University

Jeff C. Long - Department of Human Genetics, University of Michigan Medical School

Stephen T. McGarvey - International Health Institute, Brown University

Michael V. Osier - Department of Genetics, Yale University School of Medicine

Salmo Raskin - Laboratorio Genetika, Curituba, Brazil

Robert W. Robin - National Institute on Alcohol Abuse and Alcoholism, NIH

Nyamkhishig Sambuughin - Barrow Neurological Institute, St. Joseph’s Hospital and Medical Center

Guangyun Sun - Center for Genome Information, University of Cincinnati

Noreen C. Tuross - Smithsonian Center for Materials Research and Education

Yixi Zhong - School of Public Health, University of Texas Health Science Center

E. Advisees

Graduate students:

Oriana Batista - Universidad de Panama

Bradlee Alicea, Ben Burkley, Rebecca Gray Drew Kitchen, Amy Non, Nicole Nowak - Univ of Florida

Lance Gravelee - University of Michigan

Postdoctoral fellows:

Larissa Tarskaia, Angelica Gonzalez-Oliver, Marina Ascunce - University of Florida

F. Graduate and postdoctoral advisors

Dieter Soll - Molecular Biophysics and Biochemistry, Yale University

Noreen C. Tuross - Department of Anthropology, Harvard University

Eldredge Bermingham - Smithsonian Tropical Research Institute, Smithsonian Institution