Research Description:

Genetic Variation in Buffalo Clover (Trifolium reflexum) using Random Amplified Polymorphic DNA Markers (RAPDs)

Buffalo clover (Trifolium reflexum) is a rare species native to eastern North America.  Conservation of this species can be aided by knowing the degree of genetic diversity within the species.  The purpose of this research is to determine the degree of genetic variation among four geographically distinct populations of T. reflexum, and also the phylogenetic relationship between this species and at least two other Trifolium species (T. pratense and T. wormskioldii).  This goal will be met by use of random amplified polymorphic DNA (RAPD) analysis. Genomic DNA from the three species in question is being screened with a number of arbitrary 10 base-pair primers for polymorphic gel electrophoretic banding patterns.  Finding enough suitable primers probably represents the single most time-consuming aspect of the whole research plan.    In the course of screening these primers, progress has been made in optimizing the polymerase chain reaction (PCR) conditions for these species.  I have familiarized myself with the use of specialized software and imaging tools used to digitize images of gels and allow computerized analysis of RAPDs.  Finally, in a countless number of ways, I have practiced and refined my abilities to perform the multifarious everyday lab procedures necessary for my work.