Research Description:

Oltipraz Regulation of MnSOD in Intestinal Cells

I have been studying the effects of oltipraz on the levels of MnSOD in  the fetal rat intestinal cell line IRD-98.  The physiological levels of  inflammatory bowel disease (IBD) in human patients has been duplicated in  these cells.

At the start of this summer be began working with the cell line IEC-6,  however the levels of induction did not vary enough so I changed to the  IRD-98 cell line.  Since this time we have performed a dose curve to determine the optimal level of treatment for these cells.  This treatment  level was found to be 50 uL.  We are next going to perform a time curve with  this dosage amount to determine how long the cells should be treated for.

Several procedures have been used and will continue to be used in this experiment.  First, the cells were plated out and grown for approximately two days.  The cells were then treated with the oltipraz.  RNA was extracted from the cells and a northern gel was then run for 16 hours.  I then transfered  the RNA from the gel to a membrane.  At this point the membrane was hybridized with P32.

Currently, lab rats are being breed and the pups will be raised away from the parents.  These rats will be infected with colitis and treated with oltipraz.  After this occurs, the rats' blood and intestinal cells will most likely be investigated.